Single step BP/LR combined Gateway reactions
نویسندگان
چکیده
منابع مشابه
DNA-dependent single-step addition reactions catalyzed by Escherichia coli RNA polymerase.
The addition of a single nucleotide to a short oligonucleotide, catalyzed by RNA polymerase (nucleosidetriphosphate:RNA nucleotidyltransferase, EC 2.7.7.6) in the presence of synthetic DNA templates, has been studied. The reactions A-U + ATP leads to A-U-A and U-A + UTP leads to U-A-U occur in the presence of poly[d(A-T)], while the reactions G-C + GTP leads to G-C-G and C-G + CTP leads to C-G-...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 2013
ISSN: 1940-9818,0736-6205
DOI: 10.2144/000114101